Detailed instructions of nitric oxide (no) content detection kit abbkine

nitric oxide (NO) It is a reactive free radical , In neurotransmission 、 Immune response 、 Apoptosis and other important physiological functions play an important role .NO By L- Arginine channel NO Synthases (NOS) Synthetic , It has been identified as endothelium derived relaxing factor (EDRF) And antiplatelet substances . It is a neurotransmitter and cytotoxic substance produced by neutrophils and activated macrophages . although NO Molecular actions in biological systems are diverse , but NO The most important function is to activate guanylate cyclase .

nitric oxide （NO） Content detection kit kit components ：

• NaNO2  Standards  (1M)

• Grice reagent  I

• Grice reagent  II

• VCl3  Reagents

• ZnSO4

Experimental suggestions ：

CheKine The nitric oxide analysis kit is designed to be improved by using Griess The method is more accurate in measuring the reduction of nitrate to nitrite NO produce .

  nitric oxide （NO） Content detection kit features ：

• Determination of serum , The plasma , organization / Cell samples , Plant samples , The content of nitric oxide in urine and other biological liquids .

• Detection range ：1-100 µM.

• Fast and reliable , Use optimized VCl3 Reagents , take NO3 - Revert to NO2 - It takes less time .

• The method is simple 、 High throughput , Mix the sample with the reagent , Incubate and read optical density , It can easily and automatically measure thousands of samples .

nitric oxide （NO） Precautions in the use of content detection kit ：

• It is recommended to set several dilutions for the sample , To ensure that the reading is within the standard value range .

• To ensure the experimental effect , It is necessary to use fresh samples . If not detected immediately , Samples can be found in -80℃ Save for one month .

chart ; Standard curve for determination of nitric oxide .y Shaft for OD540,x Shaft for NO2 concentration （μM）.