Prosci human cell line I imprinting human immune research

AI Meijie ProSci Human cell line  I  Imprint background ：

Human cell line imprinting is specifically designed for proteomic studies to screen antibodies and protein expression . It carries  8  Different human cell lines , Each lane contains  15 µg  Total cellular protein . stay  4-20% SDS-PAGE  The protein is separated from the gel and transferred to the nitrocellulose membrane . The cell lines included in the imprint are  MCF7（ Mammary cancer ; Adenocarcinoma ）、HeLa（ cervix ; Adenocarcinoma ）、HT-29（ The colon ; Adenocarcinoma ）、293（ Kidneys ; With adenovirus  5 DNA  conversion ）、Hep G2（ The liver ; Hepatocellular carcinoma ） 、A-549（ lung ; Cancer ）、SK-N-SH（ brain ; Neuroblastoma ） and  PC-3（ prostate ; Adenocarcinoma ）.

AI Meijie ProSci  Human cell line  I  Imprint chemical property ：

species ： human beings

Loaded samples ： lane ：1、 Protein labeling ;2、MCF7;3、 Hella ;4、HT-29;5, 293; 6、Hep G2;7、A-549;8、SK-N-SH; and  9,PC-3.

The test application ：WB

Application instructions ： For Western blotting , Imprinting can be used  1% BSA  or  5%  Skimmed milk powder is sealed  1  Hours . First antibody incubation requires  30  Minutes to  2  Hours , The second antibody needs to be incubated at room temperature  30  Minutes to  1  Hours .

chart ： stay  12% SDS-PAGE  Human cell lysates were stained with Coomassie blue . lane ：1、 Protein labeling ;2、MCF7;3、 Hella ;4、HT-29;5, 293; 6、Hep G2;7、A-549;8、SK-N-SH; and  9,PC-3.

AI Meijie ProSci  Human cell line  I  Imprint characteristic ：

Percentage of gel ：

The gradient  4-20%

Storage conditions ：

Store in  4°C. The imprint can be reused after stripping .