Human cell research: prosci LAG-3 recombinant protein scheme

AI Meijie ProSci LAG-3 Recombinant protein background ：

Human lymphocyte activation gene 3 protein (LAG3) It's immunoglobulin (Ig) Members of the superfamily .LAG3  contain  4  Extracellular  Ig  Like domain .LAG3  Gene inclusion  8  Exons .LAG3  Participate in lymphocyte activation , But with  HLA II  Antigen like binding . It's activated  T  Cells and  NK  Selectively expressed in cells .LAG3  stay  T  There is negative regulation function in cells , Can be used as  T  Cell induced  B  New markers of cell activation . As a soluble molecule ,LAG3  adopt  MHC II  Signal like activation of antigen presenting cells . It can lead to antigen specificity in the body  T  Increased cellular response .LAG-3  Yes  MHC II  The affinity of class is higher than  CD4.

ProSci LAG-3 Recombinant protein chemical property ：

species ： human beings

Source species ： Human cells

Recombinant protein sequence ：Leu23-Gly434

Fusion label ：C-6 His

Application instructions ： The recombinant protein can be used in bioassay . For research purposes only .

Estimated molecular weight ：45.5 kD

AI Meijie ProSci LAG-3 Recombinant protein characteristic ：

The purity ： By reduction  SDS-PAGE  Be sure to be greater than  95%.

LAL  The endotoxin level determined by the test is lower than  0.1 ng/ug (1 IEU/ug).

Physical state ： Freeze drying

buffer ： from  20mM PB、150mM NaCl、pH 7.4  Of  0.2 um  Freeze dry the filtered solution . It is not recommended to re dissolve it below  100 ug/ml  The concentration of . Dissolve the lyophilized protein in  ddH2O  in .

Storage conditions ： Freeze dried protein should be stored  -20°C, But it can be stored stably at room temperature  3  Zhou . Recombinant protein solution is available in  4-7°C  Save  2-7  God . Aliquots of the re dissolved sample are  -20°C  It can be stored stably under  3  Months .