Worthington deoxyribonucleic acid and related research tools

Deoxyribonucleic acid  (DNA)  It is a double stranded helical nucleic acid molecule , It consists of nucleotide monomers , Each nucleotide monomer consists of a deoxyribose 、 A phosphate group and one of the four nitrogenous bases ： adenine 、 cytosine 、 Guanine and thymine .

DNA Structure and function ：

Each chromosome contains a long  DNA  molecular , Hundreds or thousands of genes are arranged along its length .DNA The molecule is a double helix , There is sugar on the outside - Phosphate bond , There are base pairs inside . Base pairing is as follows ： adenine  (A)  With thymine  (T), Guanine  (G)  With cytosine  (C).DNA  Semi reserved replication , need  RNA  primer . When the parent chain is separated , Each chain is used as a template for manufacturing new complementary chains . E. coli DNA  Replication is semi discontinuous ; One chain replicates continuously , Another discontinuous replication . This discontinuous replication forms the Okazaki fragment . E. coli proofread by  DNA  Polymerase  III  Conduct , It uses only base paired nucleotides as primers , If there is an error in blending , Will stop  DNA  Copy . At this stage ,DNA  Polymerase  III  Whole enzyme  3'  To  5'  Exonuclease can remove the wrong base . Mammalian cells have five types of polymerase ：α、β、δ、ε and γ.alpha  and  delta  Participate in two  DNA  Copy of chain , and  beta  and  epsilon  stay  DNA  Play a role in repair . Gamma is thought to replicate mitochondria  DNA (Weaver 2008).

Worthington  Deoxyribonucleic acid With a variety of  DNA, Include ：

1. From calf thymus  DNA  use  Worthington  The developed method of preparation and purification , Compared with most other commercial preparations , Protein and  RNA  Lower pollution . This highly aggregated  DNA  It's deoxyribonuclease J Good substrate . Sodium salt , It must be converted into magnesium form to  DNase  sensitive .

2.DNA、 cellulose

3.DNA, Salmon testicles

4.DNA, E. coli

5.DNA,λ, E. coli W8850（λ C1857-57）

6.DNA、λ、BstEII  fragment

7.DNA、λ、EcoRI  fragment

8.DNA、λ、HindIII  fragment

Technical specification ： A pair of bases is about 680  Dalton . One  A 260 The unit is equivalent to  50 µg DNA  product .

AI Meijie Worthington Deoxyribonucleic acid Relevant research tools ：

Albumin , Nuclease free  (BSAF)

Deoxyribonuclease  I (DP/D/DCLS/D2/DPFF/DPRF)

Deoxyribonuclease  II (HDA/HDAC/HDACS)

E•RASE RNase A/T1  Mixture  (RCT)

Histone  (H, NHL)

Lysozyme  (LY/LYSF)

Nuclease , Micrococcus  (NFCP)

Nuclease ,S1 (SINUC/SINUCL)

Phosphatase , alkalinity  (CAP/BAPF/BAPC/BAPSF/PC)

Phosphodiesterase  I (VPH)

Phosphodiesterase  II (SPH)

protease  K (PROK/PROKS)

Reverse transcriptase , restructuring  HIV (RTHIV)

Ribonuclease  A (R/RAF/RASE/RS/RPDF)

Ribonuclease  T1, No animal origin  (RT1S)

AI Meijie Worthington Deoxyribonucleic acid related literature ：

1.Aktipis, S.：DNA -  Copy process and repair , textbook  Biochem. With Colin . The correlation , The first  3  edition ,, , 767, 1992

2.Albertini, J.  and  Garnier-Suillerot, A.： iron - Bleomycin - DNA system . Deoxyribonucleic acid and β- Aminoalanine moiety α- Evidence of amino interaction  , Biochemistry 23 , 47, 1984

3.Alberts, B.： Prokaryote  DNA  Replication mechanism , Phil Trans R Soc Lond 317 , 395, 1987

4.Alberts, B.  and  Herrick, G.： Enzymology method volume .21  year ,S.Colowick  and  N. Kaplan, Academic Press , New York ,198  year ,1971  year

5.Antonarakis, S.： restructuring  DNA  The role of technology in the diagnosis of human genetic diseases , Clin Chem 35, B4,1989