Preparation of hemoglobin albumin nanoparticles encapsulated by Lumbrokinase albumin nanoparticles / erythrocyte membrane

Today, I will share with you Lumbrokinase albumin nanoparticles / The erythrocyte membrane is directionally wrapped with hemoglobin - Albumin nanoparticles Research preparation , Study with Xiaobian ！

Preparation of Lumbrokinase albumin nanoparticles ：

With p-methylbenzene sulfonyl -L- Arginine methyl ester hydrochloride (TAME) Determine the activity of Lumbrokinase for substrate , Evaluate the quality through the encapsulation efficiency . result : Determination of albumin RSD All less than 0.3%, high , in , The average recovery of low concentration is (99.47±0.93)%,(98.51±1.42)%,(98.69±2.43)%; The determination method of Lumbrokinase is high , in , Low concentration RSD Respectively 0.62%,0.37%,1.82%, The average recoveries are (99.25±1.98)%,(97.61±1.13)%,(97.15±2.21)%. The amount of Lumbrokinase albumin nanoparticles is (4.84±0.41)%, The encapsulation rate is (84.14±3.41)%.

The erythrocyte membrane is directionally wrapped with hemoglobin - Preparation of albumin nanoparticles ：

Albumin nanoparticles containing hemoglobin were prepared by solvent evaporation (Hb/BSA-NP), Then, it is prepared by physical extrusion RBC-Hb/BSA-NP, And its particle size ,Zeta Electric potential and appearance morphology ; The best dosage of erythrocyte membrane was selected by evaluating the integrity of erythrocyte membrane coated albumin nanoparticles ; Determine the correct orientation of erythrocyte membrane by measuring the sialic acid content on the surface of the preparation ; Fluorescence microscope and flow cytometry were used to detect RBCHb/BSA-NP Anti macrophage phagocytosis in vitro ; Finally, the long-term circulation effect of nano preparations in vivo is evaluated through in vivo experiments . Results prepared RBC-Hb/BSA-NP The average particle size is (127.7±3.5)nm, Average Zeta Potential is (17.1±0.28)mV, With clear nucleus - Shell structure ,72 h Good internal stability .

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Ruixi YWX.2022.7.20