Worthington core enzyme deoxyribonuclease I

Worthington Core enzymes include ： Collagenase , Deoxyribonuclease I, Elastase , Papain , Ribonuclease , Trypsin, etc . Let's take a look Worthington  Core deoxyribonuclease  I.

Worthington   Core deoxyribonuclease  I background ：

Bovine pancreatic deoxyribonuclease is an endonuclease , It preferentially cleaves the phosphodiester bond adjacent to the pyrimidine nucleotide , Produced in  3'  Having a free hydroxyl group in its position  5'- Phosphate terminated polynucleotides .DNase I  Secreted by exocrine glands , It is the highest in pancreas and parotid gland . Its content in other tissues is also low （Chen  and  Liao 2006, as well as  Nadano  wait forsomeone  1993）.

It is known that  DNase  Participate in apoptosis , It has been proposed to play a role in the regulation of actin polymerization in cells . In addition to its application in Molecular Biology ,DNase I  It is also used to treat cystic fibrosis and systemic lupus erythematosus （Chen  and  Liao 2006）.

AI Meijie Worthington Core deoxyribonuclease  I features ：

And human 、 Mice are similar to rats , Bovine pancreas  DNase I  Genes are made up of  9  Composed of exons , Only the last  8  Exons encode the protein （De María  and  Arruti 2003）. The nascent protein is encoded by exon 2  22  Amino acid signal sequences are directed to secretory pathway organelles . Main active site residues  H166  By exons  6  code （De María  and  Arruti 2003  and  Kraehenbuhl  wait forsomeone  1977）. Although the exons of cattle and other mammals are almost the same length , But the length of introns varies greatly .TATA  Box sequence in exon  I  The upstream  35 bp（De María  and  Arruti 2003）. It has been suggested that the multiple splicing events that affect the coding sequence may be down-regulation  DNase I  The mechanism of expression （Liu  wait forsomeone  1997）.

AI Meijie Worthington Core deoxyribonuclease  I works ：

bpDNase I  There are many isomers , They are different in genetics and sialic acid content （Liao 1974、Liao 1981  and  Chang  etc.  1994）. Polypeptide chain in  Asn-18  Glycosylation , And due to sialic acid in carbohydrate side chain , Shows charge heterogeneity （Chen  and  Liao 2006）.

Due to the extended hydrophobic core , The enzyme is particularly stable , The core consists of two closely packed six chains  β The folding sheet is composed of . They are surrounded by eight spirals and several ring regions , These regions are stabilized by bound calcium atoms . The enzyme passes through intramolecular hydrogen bonds 、 Salt bridge and two disulfide bonds are further stabilized （Chen  and  Liao 2006）.

AI Meijie Worthington  Core deoxyribonuclease  I application ：

1. In primary cell isolation  DNA  Remove ： Reducing viscosity provides better yield

2. In bioprocessing applications  DNA  Remove

3. stay  RT-PCR  From before  RNA  Remove the genome from the preparation  DNA

4. In vitro transcription

5. Nick translated

6.DNA Enzyme footprint

7. Actin binding

8. UV crosslinking of protein and nucleic acid

9. Radioactive marking

AI Meijie Worthington Core deoxyribonuclease  I reference ：

1.Abe, A.  and  Liao, T.： Immunological and structural comparison of deoxyribonuclease  I.  Glycosylation differences between bovine pancreas and parotid deoxyribonuclease , J Biol Chem 258 , 10283, 1983

2.Allfrey, V.  and  Mirsky, A.: J Gen Physiol 36 , 163, 1949

3.Ayvazian, J.  and  Ayvazian, L.： Effect of intravenous administration of crystalline pancreatic deoxyribonuclease on patients with gout , N Engl J Med 263 , 999, 1960

4.Ayvazian, J.、Johnson, A.  and  Tillet, W.： Pancreatic deoxyribonuclease administered parenterally is used as an adjuvant treatment for lung abscess , Amer Rev Tuberculosis Pulm Dis 76 , 1, 1957

The enzyme is Worthington Our main product line . Technically speaking ,Worthington It doesn't actually make enzymes , But from various animal and plant tissues and various microbial sources （ Like bacteria 、 Fungi and molds ） Extract enzyme from .